A water soluble bifunctional fluorogenic reagent for the sensitive and specific detection of thiols in biological specimens or proteins.
SBD-F has high reactivity to thiol compounds to attain low detection limits; no flourescence in itself; biological buffer solublility; stability at RT in water for >1 week. The optimal condition for reaction was 60oC and pH 9.5 for 1 h.
Please refer to the following references for more specific information regarding the application of SBD-F as a fluorogenic reagent for detection of cysteine containing peptides.
Fluorescence: max Abs. l = 380 nm, max Em. l = 515 nm; Molar Abs. > 4x103 (ca. 317nm)
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